Emerging RAS, BRAF, and EGFR mutations in cell-free DNA of metastatic colorectal patients are associated with both primary and secondary resistance to first-line anti-EGFR therapy.

Oncogenic RAS mutations are negative biomarkers of response to epidermal growth factor receptor (EGFR) blockade. RAS mutations are usually detected in biopsies of primary colorectal tumors. However, the genomic profiles of primary tumors and metastases are not always concordant, and chemotherapeutic agents can alter the tumor molecular landscape.

Cell-free DNA (cfDNA) is a novel tool to detect molecular heterogeneity. This study evaluated the clinical utility of cfDNA to predict primary or secondary resistance to EGFR blockade in patients with metastatic colorectal cancer. Thirty metastatic colorectal cancer patients without RAS and BRAF mutations were prospectively enrolled and treated with cytotoxic agents and EGFR blockade as first-line therapy. cfDNA was analyzed for the presence of RAS, BRAF, and EGFR (S492R) point mutations before initiating chemotherapy and every 2 months during chemotherapy.

Canine IS ELISA Kit
ECI0046 96Tests
EUR 521.00

Bovine IS ELISA Kit
EBI0046 96Tests
EUR 521.00

Chicken IS ELISA Kit
ECKI0046 96Tests
EUR 521.00

Sheep IS ELISA Kit
ESI0046 96Tests
EUR 521.00

Rat IS ELISA Kit
ERI0046 96Tests
EUR 521.00

Rabbit IS ELISA Kit
ERTI0046 96Tests
EUR 521.00

Human IS ELISA Kit
EHI0046 96Tests
EUR 521.00

Mouse IS ELISA Kit
EMI0046 96Tests
EUR 521.00

Porcine IS ELISA Kit
EPI0046 96Tests
EUR 521.00

Monkey IS ELISA Kit
EMKI0046 96Tests
EUR 521.00

Goat IS ELISA Kit
EGTI0046 96Tests
EUR 521.00

Guinea Pig IS ELISA Kit
EGI0046 96Tests
EUR 521.00

Human Serum Indoxyl Sulfate (IS) ELISA Kit
abx257961-96tests 96 tests
EUR 746.00
  • Shipped within 5-12 working days.

Human IS(serum indoxyl sulfate) ELISA Kit
EH4913 96T
EUR 567.60
  • Detection range: 31.25-2000 ng/ml
  • Alias: IS/ indoxyl sulfate/ serum indoxyl sulfate
Description: Method of detection: Coated with Antigen, Competitive ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75 ng/ml

In-situ adapter for use with T5000-96
T5000-A-IS 1 PC
EUR 2550.59
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Rabbit Phosphorylase b kinase regulatory subunit alpha, liver is
ELI-08786Ra 96 Tests
EUR 928.00

Keyhole Limpet Hemocyanin (KLH)-Agarose af is finity gel for removing KLH antibodies
KLH11-G-5 5 ml
EUR 529.00

TruStrip RDT Clenbuterol rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100010-RDT 1 pk Ask for price

TruStrip RDT Quinolone rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100020-RDT 1 pk Ask for price

TruStrip RDT Salbutamal rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100030-RDT 1 pk Ask for price

TruStrip RDT Chloramphenicol rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100040-RDT 1 pk
EUR 651.00

TruStrip RDT Sulfanethazine rapid test card, results is 2-10 mins, 50 cards/pk
DE-100100-RDT 1 pk Ask for price

TruStrip RDT Ractopamine rapid test strips, results is 2-10 mins, 50 strips/pk
DE-10020-RDT 1 pk Ask for price

TruStrip RDT Gentamicin rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100200-RDT 1 pk Ask for price

TruStrip RDT Streptomycin rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100210-RDT 1 pk Ask for price

TruStrip RDT Melamine rapid test strips, results is 2-10 mins, 50 cards/pk
DE-100270-RDT 1 pk Ask for price

TruStrip RDT Lincomycin rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100630-RDT 1 pk Ask for price

TruStrip RDT Kanamycin rapid test strips, results is 2-10 mins, 50 strips/pk
DE-850101-RDT 1 pk Ask for price

TruStrip RDT Nitrofuran (AOZ) rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100060-RDT 1 pk Ask for price

TruStrip RDT Nitrofuran (AHD rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100070-RDT 1 pk Ask for price

TruStrip RDT Nitrofuran (SEM) rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100075-RDT 1 pk Ask for price

TruStrip RDT Nitrofuran (AOZ) rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100080-RDT 1 pk
EUR 651.00

TruStrip RDT Malachite green rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100280-RDT 1 pk Ask for price

TruStrip RDT Aflatoxin B1 rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100290-RDT 1 pk Ask for price

TruStrip RDT Total Aflatoxins rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100300-RDT 1 pk Ask for price

TruStrip RDT Aflatoxin M1 rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100330-RDT 1 pk Ask for price

TruStrip RDT Sulfonamides residues (SAs) rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100090-RDT 1 pk
EUR 651.00

TruStrip RDT Beta-Lactam and Tetracyclines rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100260-RDT 1 pk Ask for price

TruStrip RDT Cow/Bovine Infectious rhinitis (IBR) antibody rapid test card, results is 2-10 mins, 50 cards/pk
RV-500300-RDT 1 pk Ask for price

TruStrip RDT Canine distemper virus (hardpad disease) antigen rapid test card, results is 2-10 mins, 50 cards/pk
RV-501200-RDT 1 pk Ask for price

The analysis was performed in 223 plasma samples from all 30 patients. Of the 30 patients, five had RAS mutations in their cfDNA before starting chemotherapy and did not respond. Twenty-four of the remaining 25 patients without cfDNA RAS mutations had a response. Twenty of the 24 responders developed secondary resistance and cfDNA RAS mutations were found in 17 of the 20. cfDNA BRAF mutations were found in seven, and EGFR mutations were found in eight of the 20 patients.

Emerging RAS, BRAF, and EGFR mutations occurred in patients with primary and secondary resistance to EGFR blockade. The detection of these mutations in cfDNA is a promising approach to predict treatment response and secondary resistance.

Scroll to Top