Lassa virus, which is a member of the Arenaviridae family of viruses, causes acute viral hemorrhagic fever. It was first reported in 1969 in the town of Lassa, Borno State, Nigeria. The natal mouse of various mammals is the main reservoir for the Lassa virus Recombinant, which is found in sub-Saharan Africa. The virus is transmitted through contact with animal faeces or urine, which contributes to its high incidence rate. Lassa fever usually occurs in West Africa. Lassa fever results in between 300,000 and 500,000 cases per year and causes about 5,000 deaths each year.
Outbreaks of this disease have been observed in Nigeria, Liberia, Sierra Leone, Guinea and the Central African Republic. Protein Structure: The Lassa virus genome is composed of two single-stranded RNA molecules defined as small (S) and large (L). Two genes in the S segment encode the nucleoprotein (NP) and two envelope glycoproteins (GP1 and GP2); while the L segment encodes the viral polymerase (L protein) and the RING finger matrix protein Z. The GP1 subunit plays a putative role in receptor binding, while the structure of the GP2 subunit is consistent with viral transmembrane fusion proteins. NP is a virion protein that binds and protects viral RNA.
Recombinant Lassa Glycoprotein-1 derived from E.Coli (Mouse/Sierra Leone/Josiah/1976 strain) containing 205 amino acids, has an Mw of 23 kDa and the isoelectric point is 6.7. Lassa GP1 protein is fused with a 6xHis tag at the C-terminus and purified using a proprietary chromatographic technique.
Fountain: Escherichia coli.
Physical Appearance: Sterile filtered solution.
Lassa GP1 Protein Solution contains phosphate-buffered saline, 100 mM arginine, and 3 M urea.
Although Lassa GP1 protein is stable at 4°C for 1 week, it should be stored below -18°C Avoid freeze-thaw cycles.
Lassa GP1 is >90% pure as determined by 10% PAGE (Coomassie stain).
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